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Syn_proteomics-hydrogen-production.xlsx (367.42 kB)

Synechocystis Proteomic data (H2 production)

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posted on 2018-02-26, 10:09 authored by Andrew Landels
This dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigating H2 production in Synechocystis sp. PCC6803" http://etheses.whiterose.ac.uk/id/eprint/19034

Synechocystis sp. PCC6803 was grown in two media conditions, standard BG11 and Burrows media; in two different head-space gas mixtures, air and 100% nitrogen (anaerobic). The experiment was continued until hydrogen gas was detected in the head-space, then the samples were collected by centrifugation and flash-frozen in liquid nitrogen. Proteomic analysis with iTRAQ labels was performed, the data was analysed with the EasyProt software (https://doi.org/10.1016/j.jprot.2012.12.012).

The first sheet of this dataset contains the full list of all proteins identified in this experiment, and the proceeding pages contain lists of proteins that were differentially regulated with statistical significance under the different environmental conditions (media and headspace).

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EU FP7 308518

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