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Densitometry analysis of Synechocystis proteins

dataset
posted on 26.02.2018 by Andrew Landels
This dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigating H2 production in Synechocystis sp. PCC6803" http://etheses.whiterose.ac.uk/id/eprint/19034

Proteins from BG11 and Burrows media were analysed by densitometry, using the software imageJ to calculate values for the different protein lanes - briefly, areas were drawn around regions on the plot and the density of the shading of the pixels was calculated in these regions. The gel image used to generate this data is included in this online data repository. The outputted densitometry values are reported in the csv file. The code in this file is written in R, and uses the package ggplot2; initially the code imports the data, finds the sums the regional densities from each of lanes on the gel, then generates a bar chart from these values.

Funding

EU FP7 308518

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