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How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls
dataset
posted on 2021-01-29, 10:51 authored by Guangyu Chen, Nathan Adams, Philip JacksonPhilip Jackson, Mark DickmanMark Dickman, Christopher HunterChristopher HunterData-files acquired by reverse phase liquid chromatography coupled online to electrospray-ionisation mass spectrometry (Q Exactive HF, Thermo Scientific).
(1) 20200213_PJ_GC_MS1: full-scan MS, 500-700 m/z
(2) 20200213_PJ_GC_PRM: product ion scans by parallel reaction monitoring for cyclase substrate MgPME, product DV PChlide a, and catalytic intermediates 131-hydroxy-MgPME and 131-keto-MgPME at m/z 598, 611, 614 and 612 respectively.
Thermo RAW files are included in mzML format which is readable by open source applications such as SeeMS (http://proteowizard.sourceforge.net/download.html).
Funding
Engineering new capacities for solar energy utilisation in bacteria
Biotechnology and Biological Sciences Research Council
Find out more...Mass spectrometry underpinning synthetic biology, industrial biotechnology and world class bioscience
Biotechnology and Biological Sciences Research Council
Find out more...History
Ethics
- There is no personal data or any that requires ethical approval
Policy
- The data complies with the institution and funders' policies on access and sharing
Sharing and access restrictions
- The data can be shared openly
Data description
- The file formats are open or commonly used
Methodology, headings and units
- Headings and units are explained in the files
