1/1
3 files

How the O2-dependent Mg-protoporphyrin monomethyl ester cyclase forms the fifth ring of chlorophylls

dataset
posted on 29.01.2021, 10:51 by Guangyu Chen, Nathan Adams, Philip Jackson, Mark Dickman, Christopher Hunter
Data-files acquired by reverse phase liquid chromatography coupled online to electrospray-ionisation mass spectrometry (Q Exactive HF, Thermo Scientific).
(1) 20200213_PJ_GC_MS1: full-scan MS, 500-700 m/z
(2) 20200213_PJ_GC_PRM: product ion scans by parallel reaction monitoring for cyclase substrate MgPME, product DV PChlide a, and catalytic intermediates 131-hydroxy-MgPME and 131-keto-MgPME at m/z 598, 611, 614 and 612 respectively.

Thermo RAW files are included in mzML format which is readable by open source applications such as SeeMS (http://proteowizard.sourceforge.net/download.html).

Funding

Engineering new capacities for solar energy utilisation in bacteria

Biotechnology and Biological Sciences Research Council

Find out more...

Redesigning the Photosynthetic Light Reactions

European Research Council

Find out more...

Mass spectrometry underpinning synthetic biology, industrial biotechnology and world class bioscience

Biotechnology and Biological Sciences Research Council

Find out more...

History

Ethics

There is no personal data or any that requires ethical approval

Policy

The data complies with the institution and funders' policies on access and sharing

Sharing and access restrictions

The data can be shared openly

Data description

  • The file formats are open or commonly used

Methodology, headings and units

  • Headings and units are explained in the files

Licence

Exports