The University of Sheffield
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HEK293T CBP-HaloTag clonal line single molecule imaging using different exposure times.

posted on 2023-09-08, 10:18 authored by Katie GelderKatie Gelder

3 videos showing single molecules of CBP from clonal HEK293T cell line containing a CBP-HaloTag which was labelled with Janelia Fluor 646 (Promega). Images were taken with 100 % laser power on custom TIRF microscope with the following exposure times: 10 ms, 20 ms and 50 ms. Videos were made in ImageJ/Fiji so that all of the images have the right number of frames/second (e.g. 10 ms has 100 fps, 20 has 50 fps).


BBSRC White Rose Mechanistic Biology DTP



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    Department of Molecular Biology and Biotechnology