Densitometry analysis of Synechocystis proteins
software
posted on 2018-02-26, 10:10 authored by Andrew LandelsThis dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigating H2 production in Synechocystis sp. PCC6803" http://etheses.whiterose.ac.uk/id/eprint/19034
Proteins from BG11 and Burrows media were analysed by densitometry, using the software imageJ to calculate values for the different protein lanes - briefly, areas were drawn around regions on the plot and the density of the shading of the pixels was calculated in these regions. The gel image used to generate this data is included in this online data repository. The outputted densitometry values are reported in the csv file. The code in this file is written in R, and uses the package ggplot2; initially the code imports the data, finds the sums the regional densities from each of lanes on the gel, then generates a bar chart from these values.
Proteins from BG11 and Burrows media were analysed by densitometry, using the software imageJ to calculate values for the different protein lanes - briefly, areas were drawn around regions on the plot and the density of the shading of the pixels was calculated in these regions. The gel image used to generate this data is included in this online data repository. The outputted densitometry values are reported in the csv file. The code in this file is written in R, and uses the package ggplot2; initially the code imports the data, finds the sums the regional densities from each of lanes on the gel, then generates a bar chart from these values.
Funding
EU FP7 308518
History
References
Ethics
There is no personal data or any that requires ethical approvalPolicy
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