230 by 260 OD test table.xls (12.5 kB)
Download fileKalb protein quantification test
software
posted on 2018-02-26, 10:10 authored by Andrew LandelsThis dataset/code forms part of Andrew Landels' thesis: "Improving proteomic methods and investigating H2 production in Synechocystis sp. PCC6803" http://etheses.whiterose.ac.uk/id/eprint/19034
A protein quantification method, devised by Kalb and Bernlohr, was utilised to remove the influence of phycobilisome pigments on protein quantification prior to analysis. Control samples of bovine serum albumin (BSA) and bovine cytochrome c (cyt) were used as standards of known concentration to investigate this method and how it scaled for individual proteins and mixtures of proteins.
This dataset shows that there was an over-estimation of cyt that scaled linearly, and a slight underestimation of BSA that did not appear to scale. When combined together, the effects of the linear scaling were still present, however were reduced by half.
A protein quantification method, devised by Kalb and Bernlohr, was utilised to remove the influence of phycobilisome pigments on protein quantification prior to analysis. Control samples of bovine serum albumin (BSA) and bovine cytochrome c (cyt) were used as standards of known concentration to investigate this method and how it scaled for individual proteins and mixtures of proteins.
This dataset shows that there was an over-estimation of cyt that scaled linearly, and a slight underestimation of BSA that did not appear to scale. When combined together, the effects of the linear scaling were still present, however were reduced by half.
Funding
EU FP7 308518
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